Title : Innovative approach for beetroot (beta vulgaris l.) aqueous extraction by cyclodextrins and its use to alleviate ethanol induced gastric ulcer in rats
Abstract:
Gastric ulcer is considered one of the high-risk diseases linked with the current lifestyle, while supplementation of natural antioxidants could affect the progression of gastric ulcer. In the current work, a novel aqueous extraction of beetroot, as a source of natural antioxidants, was used for effective mitigation of gastric ulcer in rats as a way for human health promotion suggestion. For the first time, different amounts of β-CD and HP-β-CD ranged from 0.5-7% (w/w) were used for developing the beetroot extraction efficiency in the presence of stirring and ultrasound emerged technology. It was found that the highest total phenolic compounds and total betanin values (3.1 mcg/mL and 3.01 mg/mL, respectively) were achieved in HP-β-CD solution. After that, the effect of modified beetroot extract on ethanol induced Gastric Ulcer in male albino rats was studied. Serum malondialdehyde (MDA), nitric oxide (NO), serum reduced glutathione (GSH), serum tumor necrosis factor alpha (TNF-α), interleukin -6 (IL-6)), serum IL-10, serum myeloperoxidase (MPO) and serum prostaglandin E2 (PGE2) were investigated. Moreover, the histological examination of stomach was performed after treatment with beetroot extraction alone and combined with omeprazole as a reference drug. The obtained results showed that pretreatment with beetroot extract and/or the combination with omeprazole protected markedly (P≤0.0001) the gastric tissue from the damaging impact by absolute ethanol. Therefore, the proposed synergistic pretreatment approach could be used as a model for further investigation of natural antioxidants impact on several gastrointestinal (GI) diseases.
Audience Take Away:
• Improving the extraction efficiency of aqueous natural antioxidants
• Using aqueous extract of beetroot for mitigation of gastric ulcer
• Biochemical and histopathological investigation of stomach pretreated
• Suggested model for human health promotion
